Activity of Enzyme in Escherichia Coli Through Molecular Techniques
DOI:
https://doi.org/10.55606/ijhs.v5i2.4991Keywords:
Activity, Concentration, Enzyme, Escherichia Coli, SpectrophotometryAbstract
Enzymes are protein biomolecules that act as catalysts in various biochemical reactions, both in biological systems and industrial applications. The uniqueness of enzymes lies in their ability to accelerate the rate of reactions with a high degree of specificity towards certain substrates without undergoing permanent changes in their chemical structure. Enzyme activity is strongly influenced by various environmental factors, such as temperature, pH, substrate concentration, and the presence of inhibitors or activators. Therefore, quantitative testing of enzyme activity is an important step in understanding the characteristics of enzymes and their applications in various fields. Escherichia coli produces Extended-Spectrum Β-Lactamase Enzyme (ESBL) and plays a role in damaging the structure of beta lactam antibiotics so that the antibiotics cannot kill bacteria. Bacteria that produce ESBLs need to be watched out for because ESBLs are produced by genes located on plasmids, which can easily be transferred to other bacteria, and often also carry resistance genes to other antibiotics. Objective: to accurately measure the activity or concentration of enzymes in samples of Escherichia coli bacteria and understand the influence of variables such as substrate concentration on the reaction rate. Method: spectrophotometry through enzyme extraction, making a standard curve and testing enzyme activity against variations in substrate concentration. Results: samples with concentrations of 0.1 and 0.3 showed good and appropriate absorbance results. However, in the sample 0.5 ; 0.7 ; and 1.0 indicates an absorbance number that is slightly higher than it should be. Conclusion: the enzyme in Escherichia coli bacteria has good activity at sample concentrations of 0.1 and 0.3.
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References
L. Advinda and A. Fariani, "Pengaruh Berbagai Konsentrasi Sabun Padat Antiseptik Terhadap Escherichia coli," Jurnal Serambi Biologi, vol. 7, no. 3, pp. 229-234, 2022.
Z. Ahriani, Hernawati, and Fitriyanti, "Analisis Nilai Absorbansi Untuk Menentukan Kadar Flavonoid Daun Jarak Merah (Jatropha Gossypifolia L.) Menggunakan Spektrofotometer UV-Vis," Jurnal Fisika dan Terapannya, vol. 8, no. 2, pp. 56-54, 2021.
J. Akuba and M.S. Pakaya, "Uji Aktivitas Enzim Diastase Madu Hutan Mentah Gorontalo Sebagai Imunomodulator," Pharmasipha, vol. 4, no. 2, pp. 30-33, 2020.
B. Alberts, et al., Molecular Biology of the Cell, 4th ed. Garland Science, 2002.
D. Amalia, N.N. Rahmi, N. Hidayati, R. Oktaviana, Z. Aurora, B. Supriatno, and S. Anggraeni, "Pengaruh Volume Substrat Terhadap Kerja Enzim Katalase Menggunakan Respirometer Ganong Sebagai Rekonstruksi Desain Kegiatan Praktikum Siswa," Best Journal Biology Educational Science dan Technology, vol. 5, no. 2, pp. 2-17, 2022.
J.M. Berg, et al., Biochemistry, 6th ed. W.H. Freeman, 2002.
A. Cornish-Bowden, Fundamentals of Enzyme Kinetics, 4th ed. Wiley-Blackwell, 2012.
F. Damira, N. Firdha, S.A. Farma, Y. Atifah, and S. Batungale, "Activity Of The Amylase Enzyme In Saliva And The Protease Enzyme In The Rana esculenta Pancreatic Secretions," Prosiding SEMNAS BIO Universitas Negeri Padang, vol. 1, pp. 111-121, 2021.
V. Deavina, A. Rachmawati, and E. Yulianti, "Uji Aktivitas Enzim Protease Bakteri Termofilik Pasca Erupsi Merapi Terhadap Tepung Jeroan Ikan Lele," Jurnal Prodi Pendidikan Biologi, vol. 7, no. 6, pp. 398-402, 2018.
V. Deviana and A. Rakhmawati, "Uji Aktivitas Enzim Protease Bakteri Termofilik Pasca Erupsi Merapi Terhadap Tepung Jeroan Ikan Lele," Jurnal Prodi Pendidikan Biologi, vol. 7, no. 6, pp. 398-402, 2018.
R.K. Dewi and I. Prihatini, "Kandungan Enzim Papain pada Pepaya (Carica papaya L.) Terhadap Metabolisme Tubuh," Jurnal Tadris IPA Indonesia, vol. 1, no. 3, pp. 449-558, 2021.
M.H.E. Effendi, "Kejadian Extended-spectrum β-lactamase (ESBL) yang Diproduksi Escherichia coli dari Peternakan," 2020. [Online]. Available: https://unair.ac.id/kejadian-extended-spectrum-%CE%B2-lactamase-esbl-yang-diproduksi-escherichia-coli-dari-peternakan/
N. Fitriana and M.T. Asri, "Aktivitas Proteolitik pada Enzim Protease dari Bakteri Rhizosphere Tanaman Kedelai (Glycine max L.) di Trenggalek," Lentera Bio, vol. 11, no. 1, pp. 144-152, 2022.
Gumelar and D.E. Fariyanto, "Pengaruh Waktu Perkecambahan Biji Kacang Hijau (Phaseolus Radiatus L.) Terhadap Produksi Enzim α-Amilase," Cermin: Jurnal Penelitian, vol. 4, no. 1, pp. 68-77, 2020.
M.A. Harder and M. Schmidt, "Impact of Environmental Factors on Enzyme Activity," Environmental Science & Technology, vol. 40, no. 9, pp. 345-358, 2016.
N.W.N. Hasanah and M. Ilmi, "Penapisan Enzim Invertase dari Khamir Asal Nektar dan Madu Hutan," Majalah Ilmiah Biologi Biosfera: A Scientific Journal, vol. 37, no. 3, pp. 141-146, 2020.
G.N. Inayah, A. Rahamadayanti, A. Argiyanti, R.I. Sukma, B. Supriatno, and S. Anggraeni, "Alternatif Kegiatan Praktikum Tingkat SMA: Pengaruh pH terhadap Hasil Kerja Katalase Menggunakan Respirometer Ganong," Edukatif: Jurnal Ilmu Pendidikan, vol. 4, no. 4, pp. 5422-5444, 2022.
E. Indrawati, A. Susilowati, D.P. Atmojo, and N. Mulyana, "Efektivitas Enzim Kasar Kitinase Dari Jamur Trichoderma viride Yang Diiradiasi Oleh Sinar Gamma Terhadap Degradasi Cangkang Telur Nematoda Haemonchus contortus Pada Ternak Domba," Jurnal Ilmu-Ilmu Peternakan, vol. 29, no. 1, pp. 24-36, 2018.
D. Isti’anah, U. Utami, and A. Barizi, "Karakterisasi Enzim Amilase dari Bakteri Bacillus megaterium pada Variasi Suhu, pH dan Konsentrasi Substrat," Jurnal Riset Biologi dan Aplikasinya, vol. 2, no. 1, pp. 11-17, 2021.
A. Kusumaningrum, I.B.W. Gunam, and I.M.M. Wijaya, "Optimasi Suhu Dan pH Terhadap Aktivitas Enzim Endoglukanase Menggunakan Response Surface Methodology (RSM)," Jurnal Rekayasa dan Manajemen Agroindustri, vol. 7, no. 2, pp. 243-253, 2019.
Y. Liu and Z. Chen, "Spectrophotometric Determination of Amylase Activity," Journal of Applied Biochemistry, vol. 33, no. 3, pp. 123-129, 2007.
R.R.P. Megahati, "Uji Aktivitas Enzim Penislin G Asilase (Pga) Secara Kualitatif Terhadap Isolat Escherichia coli Yang Berasal Dari Air Sungai Di Kota Padang," Jurnal Pelangi, vol. 4, no. 2, pp. 154-161, 2011.
H. Muliasari and L. Permatasari, "Studi Awal Uji Aktivitas Enzim Amilase Dari Tumbuhan Secara Kualitatif Berdasarkan Perbedaan Suhu dan Konsentrasi Substrat," Journal of Agritechnology and Food Processing, vol. 2, no. 1, pp. 29-34, 2022.
H. Murtiyaningsih and M. Hazmi, "Isolasi Dan Uji Aktivitas Enzim Selulase Pada Bakteri Selulolitik Asal Tanah Sampah," Agritrop, vol. 15, no. 2, pp. 293-308, 2017.
D.L. Nelson and M.M. Cox, Lehninger Principles of Biochemistry, 5th ed. W.H. Freeman, 2008.
Nurkhotimah and E. Yukianti, "Pengaruh Suhu Dan pH Terhadap Aktivitas Enzim Fosfatase Bakteri Termofilik Sungai Gendol Pasca Erupsi Merapi," Jurnal Prodi Biologi, vol. 6, no. 8, pp. 465-471, 2017.
E.M. Prantantie, V.P. Bintoro, and B. Dwiloka, "Isolasi Enzim Amilase Dari Kecambah Kacang Tunggak (Vigna unguiculata)," Jurnal Ilmiah Teknosains, vol. 7, no. 1, pp. 29-35, 2021.
W.A. Putra, R. Karnila, and A. Diharmi, "Aktivitas Ekstrak Kasar Enzim Kolagenase Dari Organ Dalam Ikan Malong (Congresox talabon) Pada pH Berbeda," Jurnal Teknologi dan Industri Pertanian Indonesia, vol. 13, no. 1, pp. 27-30, 2021.
D.M. Putri, L. Ristiani, and Q. Hasanah, "Peran Enzim dalam Proses Metabolisme Menurut Al-Quran dan Hadist," ISTISYFA: Journal of Islamic Guidance and Counseling, vol. 2, no. 1, pp. 194-206, 2023.
D.K. Sari, A. Permanasari, and F.M.T. Supriyanti, "Pemanfaatan Material Lokal sebagai Bahan Praktikum Kinetika Enzim untuk Meningkatkan Ketrampilan Berpikir Kreatif Mahasiswa Calon Guru Kimia," Prosiding SNIPS, ISBN: 978-602-61045-0-2, 2016.
I.P. Sari and Indrayani, "Karakterisasi Enzim Amilase Dari Isolat Khamir Hasil Fermentasi Biji Kopi Robusta (Coffea canephora)," Jurnal Pendidikan Teknologi Pertanian, vol. 10, no. 1, pp. 39-52, 2024.
C.J.K. Simamora and Sukmawati, "Identification and Characterization of PrTK-2 Bacterial Isolate Producing Extracellular Protease Enzym from Rubber Seeds Tempeh," Bioscience, vol. 2, no. 1, pp. 79-88, 2018.
C.J.K. Simamora and Sukmawati, "Identifikasi dan Karakterisasi Aktivitas Ekstrak Kasar Enzim Lipase Isolat Bakteri Lipolitik Lptk 19 Asal Tempe Biji Karet," Median, vol. 12, no. 1, pp. 28-37, 2020.
H. Solahuddin, N.I. Hanifa, R. F. Deccati, and H. Muliasari, "Isolasi dan Uji Aktivitas Enzim Selulase dari Rumen Sapi (Bibos javanicus)," Journal of Science, Technology and Entrepreneurship, vol. 3, no. 1, pp. 1-7, 2021.
F.D. Sulistiyono, L. Soesanto, and N.I. Ratnaningtyas, "Uji Aktivitas Protease Empat Isolat Trichoderma spp. yang Berasal dari Tanah Perakaran," Chimica et Natura Acta, vol. 9, no. 3, pp. 98-101, 2021.
S. Sumardi, S. Farisi, C.N. Ekowati, and M.S. Diana, "Aktivitas Dan Karakterisasi Enzim Protease Isolat Bacillus Sp. (Uj132) Secara Kualitatif Dan Kuantitatif," Jurnal Riset Akuakultur, vol. 14, no. 3, pp. 193-199, 2019.
H.M. Vera, W. Astuti, and D.R. Pratiwi, "Skrining Lipase Dari Bakteri Air Bendungan Benanga Lempake Kecamatan Samarinda Utara Dan Potensinya Sebagai Bahan Aditif Detergen," Jurnal Atomik, vol. 8, no. 2, pp. 50-53, 2023.
A. Yusron, N. Purwitasari, and H. Abdillah, "Uji Aktivitas Enzim Protease Pada Kedelai Grade C Yang Difermentasi Padat Dengan Inokulum Tempe Kediri," Simposium Nasional RAPI XX FT UMS ISSN 2686-4274, 2021.
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