Chemometric Analysis of Phytochemical Content and Antioxidant Activity of Ethanol Extract 70% and Ethyl Acetate of Kersen Leaf (Muntingia calabura L.)

Afif Putri Habibah; Endang Setyowati; Emma Jayanti Besan

doi:10.55606/ijhs.v5i2.5583

Authors

Afif Putri Habibah Universitas Muhammadiyah Kudus
Endang Setyowati Universitas Muhammadiyah Kudus
Emma Jayanti Besan Universitas Muhammadiyah Kudus

DOI:

https://doi.org/10.55606/ijhs.v5i2.5583

Keywords:

chemometrics, DPPH, Muntingia calabura L.flavonoids, phenolics

Abstract

This study analyzed the phytochemical content and antioxidant activity of kersen leaf extract (Muntingia calabura L.) obtained with 70 % ethanol and ethyl acetate through a chemometric approach. Extraction is carried out by maceration, total flavonoids are determined by UV-Vis spectrophotometry with quercetin, while total phenolics with gallic acid. Antioxidant activity was tested using the DPPH method and the IC₅₀ value was calculated. The results showed that the total flavonoid content in 70% ethanol extract was 3.374 ± 0.082 mg EK/mL, while in ethyl acetate extract it was 2.726 ± 0.014 mg EK/mL. The total phenolic content in 70% ethanol extract was 152.34 ± 5.71 mg EAG/mL, while in ethyl acetate extract it was 262.78 ± 6.27 mg EAG/mL. The highest antioxidant activity was obtained from ethyl acetate extract with an IC₅₀ value of 34.45 ± 1.23 μg/mL, while ethanol extract 70% showed an IC₅₀ value of 42.35 ± 7.22 μg/mL. Chemometric analysis using Principal Component Analysis (PCA) showed a relationship between the 70% ethanol flavonoid content and ethyl acetate on the antioxidant activity of the DPPH method.

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References

Adiyasa, M. R. and Meiyanti, M., “Pemanfaatan obat tradisional di Indonesia: distribusi dan faktor demografis yang berpengaruh,” Jurnal Biomedika dan Kesehatan, vol. 4, no. 3, pp. 130–138, 2021. [Online]. Available: https://doi.org/10.18051/jbiomedkes.2021.v4.130-138

Annisa, N. and Najib, S. Z., “Skrining Fitokimia dan Penetapan Kadar Total Fenol,” Indonesian Journal of Pharmaceutical and Herbal Medicine, vol. 1, no. 2, pp. 96–104, 2022.

Apriliana, A. et al., “Comparison of the Maseration and Refluks,” Jurnal Farmasi Galenika, vol. 6, no. 1, pp. 37–38, 2020.

Arina, Y., Shiyan, S., and Suprayetno, S., “Analisis Kemometrik Ekstrak Akar Tunjuk Langit (Helminthostachys zeylanica L.) melalui Analisis Fourier Transformed Infrared dari Berbagai Daerah Sumatera Selatan,” Jurnal ’Aisyiyah Medika, vol. 7, no. 1, pp. 243–258, 2022. [Online]. Available: https://doi.org/10.36729/jam.v7i1.790

Aulani, F. N., “Cara BPOM memastikan keamanan obat tradisional di masyarakat,” Farmasetika.Com (Online), vol. 3, no. 2, p. 24, 2019. [Online]. Available: https://doi.org/10.24198/farmasetika.v3i2.21620

Besan, E. J., Fadel, M., Dahbul, N., Dameria, G. H., and Prasetyawan, F., “Uji Aktivitas Antioksidan Ekstrak dan Fraksi-fraksi Herba Suruhan (Peperomia pellucida L.) dengan Metode DPPH,” Indonesia Jurnal Farmasi, vol. 5, pp. 224–233, 2015.

BPOM RI, Farmakope Indonesia, Edisi VI, 2020.

Departemen Kesehatan Republik Indonesia, Parameter Standar Umum Ekstrak Tumbuhan Obat, 2000.

Fang, H., Zhang, H., Wei, X., Ye, X., and Tian, J., “Phytochemicals and Antioxidant Capacities of Young Citrus Fruits Cultivated in China,” Molecules, vol. 27, no. 16, 2022. [Online]. Available: https://doi.org/10.3390/molecules27165185

HDT, M., “Advancing In vitro Antioxidant Activity Assessment: A Comprehensive Methodological Review and Improved Approaches for DPPH, FRAP and H2O2 Assays,” Journal of Natural & Ayurvedic Medicine, vol. 7, no. 4, pp. 1–7, 2023. [Online]. Available: https://doi.org/10.23880/jonam-16000431

Khairunnisa, S., Hakim, A. R., and Audina, M., “Perbandingan Kadar Flavonoid Total Berdasarkan Perbedaan Konsentrasi Pelarut Etanol dari Ekstrak Daun Pegagan (Centella asiatica [L] Urban),” Journal Pharmaceutical Care and Sciences, vol. 3, no. 1, pp. 121–131, 2022. [Online]. Available: https://doi.org/10.33859/jpcs.v3i1.236

Kucharska-Ambrożej, K. and Karpinska, J., “The Application of Spectroscopic Techniques in Combination with Chemometrics for Detection Adulteration of Some Herbs and Spices,” Microchemical Journal, vol. 153, 2020. [Online]. Available: https://doi.org/10.1016/j.microc.2019.104278

Munteanu, I. G. and Apetrei, C., “Analytical Methods Used in Determining Antioxidant Activity: A Review,” International Journal of Molecular Sciences, vol. 22, no. 7, 2021. [Online]. Available: https://doi.org/10.3390/ijms22073380

Panaungi, A. N. and Hasma, “Formulasi Sediaan Sabun Cair dari Ekstrak Etanol Daun Kersen (Muntingia calabura L.),” Journal Syifa Sciences and Clinical Research (JSSCR), vol. 4, no. 3, pp. 781–782, 2022.

Puspitasari, A. D. and Syam, L. P., “Perbandingan Metode Ekstraksi Maserasi dan Sokletasi terhadap Kadar Fenolik Total Ekstrak Etanol Daun Kersen (Muntingia calabura),” Jurnal Ilmiah Cendekia Eksakta, vol. 1, no. 2, pp. 1–8, 2017.

Rahmadani, N. et al., “Tentang Etnobiologi di Kalimantan Selatan,” unpublished, March 2022.

Risal, Y., “Analisis Kemometrik Senyawa Inhibitor Tirosinase Menggunakan Spektrofotometer IR (FTIR),” Majalah Farmasi dan Farmakologi, vol. 24, no. 2, pp. 59–62, 2020. [Online]. Available: https://doi.org/10.20956/mff.v24i2.10610

Risal, Y., “Analisis Kemometrik Berdasarkan Spektra Infra Merah (IR) dan Uji Aktivitas Senyawa Inhibitor Tirosinase,” unpublished, 2022. [Online]. Available: http://repository.unhas.ac.id/id/eprint/15505/

Samodra, G., Alfathani, N. F., and Octaviani, P., “Uji Aktivitas Antioksidan Ekstrak Etanol Kombinasi Daun Kersen (Muntingia calabura L.) dan Daun Kelor (Moringa oleifera L) dengan Metode DPPH,” Pharmacon: Jurnal Farmasi Indonesia, vol. 20, no. 1, pp. 19–26, 2023. [Online]. Available: https://doi.org/10.23917/pharmacon.v20i1.22293

Setyowati, E., Ikawati, Z., Hertiani, T., & Pramantara, I. D. P. (2020). Antioxidant activity and lipase enzyme inhibition of gynura procumbens (Lour.) merr and curcuma xanthorrhiza roxb and their correlation with chemometric methods. International Journal of Pharmaceutical Research, 12(2), 2845–2854. https://doi.org/10.31838/ijpr/2020.SP2.294

Vonna, A. et al., “Kopelma Darussalam, Banda Aceh 23111, Indonesia,” Jurnal Bioleuser, vol. 5, no. 3, pp. 8–12, 2021. [Online]. Available: http://www.jurnal.unsyiah.ac.id/bioleuser

Widodo, H., Sismindari, S., Asmara, W., and Rohman, A., “Antioxidant Activity, Total Phenolic and Flavonoid Contents of Selected Medicinal Plants Used for Liver Diseases and Its Classification with Chemometrics,” Journal of Applied Pharmaceutical Science, vol. 9, no. 6, pp. 99–105, 2019. [Online]. Available: https://doi.org/10.7324/JAPS.2019.90614