Secondary Metabolite Identification and Antibacterial Activity of Ethanol Extract of Coconut Husk (Cocos nucifera L.) Against Escherichia coli ATCC 25922

Bernadeta Videlsia Ikun; Syahrir Syahrir; Nimas Prita Rahajeningtyas Kusuma Wardani; Muhajirin Dean

doi:10.55606/ijhs.v6i1.6838

Authors

Bernadeta Videlsia Ikun Universitas Nusa Cendana
Syahrir Syahrir Universitas Nusa Cendana
Nimas Prita Rahajeningtyas Kusuma Wardani Universitas Nusa Cendana
Muhajirin Dean Universitas Nusa Cendana

DOI:

https://doi.org/10.55606/ijhs.v6i1.6838

Keywords:

Antibacterial Activity, Coconut Husk, Escherichia Coli ATCC 25922, Flavonoids, Secondary Metabolites

Abstract

Secondary metabolites are bioactive compounds commonly found in plants and have potential as natural antibacterial agents. Coconut coir (Cocos nucifera Linnaeus), an abundant agricultural by-product, is known to contain various secondary metabolites such as flavonoids, tannins, terpenoids, and phenolic compounds, which have been reported to possess antibacterial properties. Escherichia coli is a pathogenic bacterium that frequently causes human infections and has shown increasing resistance to several antibiotics, highlighting the need for alternative antibacterial sources. Inconsistencies in previous studies regarding the antibacterial activity of coconut coir against E. coli indicate the necessity for further investigation. This study aimed to identify the secondary metabolite content and evaluate the antibacterial activity of ethanol extract of coconut coir against Escherichia coli ATCC 25922. This study was an in vitro laboratory experimental study with a descriptive–experimental approach. Identification of secondary metabolites was conducted qualitatively using phytochemical screening of the ethanol extract of coconut coir. Antibacterial activity was assessed using the disc diffusion method with a post-test only control group design against Escherichia coli ATCC 25922. The phytochemical screening results demonstrated that the ethanol extract of coconut coir contained flavonoids, tannins, terpenoids, and polyphenols. However, the antibacterial activity test showed no inhibition zones at all tested concentrations of the ethanol extract against Escherichia coli ATCC 25922. These findings indicate that although the ethanol extract of coconut coir contains several secondary metabolites, it does not exhibit antibacterial activity against Escherichia coli ATCC 25922 when tested using the disc diffusion method.

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References

Aniar, M., Swasono, H., Wardani, H., Utami, C. R., Aniar, M., Swasono, H., & others. (2022). The Effect of Ethanol Solvent Concentration on the Characteristics of Young and Old Coconut Coir Tannin Extracts. 53(10).

Arziyah, D., Kasim, A., Asben, A., & Busniah, M. (2025). Analysis of the Chemical Content of Coconut Husk as a Raw Material for Furfural Production. J Appl Agric Sci Technol, 9(3), 413-424. https://doi.org/10.55043/jaast.v9i3.393

Delcour, A. H. (n.d.). Outer membrane permeability and antibiotic resistance. Biochim Biophys Acta - Proteins Proteomics, 808-816. https://doi.org/10.1016/j.bbapap.2008.11.005

Harborne, J. (1998). Metode fitokimia (2nd ed.). ITB.

Hutasoit, D. P. (2020). Pengaruh Sanitasi Makanan dan Kontaminasi Bakteri Escherichia coli Terhadap Penyakit Diare. J Ilm Kesehat Sandi Husada, 12(2), 779-786. https://doi.org/10.35816/jiskh.v12i2.399

Jawetz, E., Melnick, J. L., Adelberg, E. A., Carroll, K., Morse, S., & others. (2016). Medical Microbiology (27th ed.). McGraw-Hill.

Lobo, A. M. N., Lidia, K., Nurina, L., & Indriarini, D. (n.d.). Uji Aktivitas Antibakteri Ekstrak Etanol 96.

Madigan, M. T., Bender, K. S., Buckley, D. H., Sattley, W. M., & Stahl, D. A. (2018). Brock Biology of Microorganisms (15th ed.). Pearson.

Nugraha, I. K. A., & Hendrayana, M. A. (2020). Efek ktivitas Antibakteri Ekstrak Sabut Kelapa (Cocos nucifera L.) Varietas Dalam Terhadap Pertumbuhan Bakteri Extended Spectrum β-Lactamase Producing Escherichia coli Secara In Vitro *. J Med Udayana, 9(4), 106-110.

Nurjanah, G. S., Cahyadi, A. I., & Windria, S. (2020). Escherichia Coli Resistance To Various Kinds of Antibiotics in Animals and Humans: a Literature Study. J Indones Med Veterinus, 9(6), 970-983. https://doi.org/10.19087/imv.2020.9.6.970

Organization, W. H. (2022). Report signals increasing resistance to antibiotics in bacterial infections in humans and need for better data.

Purwaningrum, N. D. (2021). Uji aktivitas antibakteri ekstrak dan fraksi n-heksan, etil asetat dan air dari sabut kelapa muda (Cocos nucifera Linn) terhadap Escherichia coli ESBL (extended spectrum beta lactamase). Sekol Tinggi Ilmu Kesehat Nas.

Rahayu, A., Hanum, F. F., Alfi, N., Amrillah, Z., & Lim, L. W. (2022). Cellulose Extraction From Coconut Coir With Alkaline Delignification Process. J Fibers Polym Compos, 1(2), 106-116. https://doi.org/10.55043/jfpc.v1i2.51

Sari, & dkk. (2021). Penentuan Kandungan Metabolit Sekunder Ekstrak Etanol Sabut Kelapa Muda (cocos nucifera L.) Secara Kualitatif. J Res Educ Chem, 3(2), 113. https://doi.org/10.25299/jrec.2021.vol3(2).7579

Tinasy, N. A., & Wijayati, N. (2024). Effects of Extraction Methods on Total Phenolic Content, Total Flavonoid Content, and Antioxidant Activity of Extract Mango Leaves (Mangifera indica L .). Indones J Chem Sci, 13(3).

Umarudin, A. (2023). IG. Bakteriologi 2. Akbar H, editor (Vol. 1). CV. MEDIA SAINS INDONESIA. Bandung.

zhi, L. X., Plésiat, P., & Nikaido, H. (2015). The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria. Clin Microbiol Rev, 358.